ANALYSIS OF MOLECULES INVOLVED IN RECEPTOR MEDIATED SIGNALING NIH GUIDE, Volume 25, Number 9, March 22, 1996 RFP AVAILABLE: NLM 96-106/DJH P.T. 34 Keywords: Proteins and Macromolecules Receptors Peptides National Library of Medicine The National Institute of Dental Research (NIDR) has a requirement to perform state-of-the-art protein sequencing on purified preparations that are available in very small quantities. The contractor shall determine, in approximately 10 proteins each year, both the amino acid composition and sequence analysis of samples that contain as little as 10 pmoles of protein. The contractor shall perform N- terminal sequencing of samples, protein digestion of samples in gels or on membranes, separation of the peptides, and identification of the peptides by either sequencing or mass spectroscopy. The proteins will be molecules involved in signal transduction in eukaryotic cells. NIDR will provide the contractor with the purified materials for these analyses. Based on either the amino acid sequence results or on the cDNA sequence supplied by NIDR, the contractor shall synthesize peptides 10-15 amino acids long. Peptides will be purified, characterized, and some coupled to two different carrier proteins. The total amount of peptide will be in the 5-to-20 mg range and approximately 10 to 20 peptides will be synthesized each year. All sequence data provided by NIDR to the contractor will be confidential. Antibodies will be required for 10 of these peptides or proteins. Two rabbits will be immunized with each purified protein or synthetic peptide. Samples of synthetic peptides and rabbit blood will be delivered, and detailed reports will be submitted regarding the results obtained from the analyses of samples. The contractor must have demonstrated expertise in methods of protein sequencing, synthesis of peptides, peptide purification, and coupling to carriers for immunization of animals. It is essential that the Government maintain rights to data and patents emanating from this requirement. The Government intends to restrict the patent rights under the awarded contract and is in the process of obtaining approval of a deviation to the standard patent rights clause (FAR 52.227-11), which will be included in any resultant contract. It is anticipated that a three-year, cost reimbursement (completion) type contract will be awarded. INQUIRIES RFP No. NLM 96-106/DJH will be available on or about April 2, 1996, with proposals due on or about May 17, 1996. All responsible sources may submit a proposal that will be considered by the NIDR. This acquisition is being managed for the NIDR by the Office of Acquisitions Management at the National Library of Medicine. Verbal and FAX requests for the RFP will not be accepted, requests must be submitted in writing, addressed to: Daniel J. Hartinger Office of Acquisitions Management National Library of Medicine 8600 Rockville Pike Building 38A, Room B1N17 Bethesda, MD 20894 .
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