PREPARATION OF GONADAL CELLS AND CELL FRACTIONS

NIH GUIDE, Volume 24, Number 15, April 28, 1995



RFP AVAILABLE:  NICHD-IRP-95-15



P.T. 34



Keywords:

  Biological Resources 

  Cell Lines 



National Institute of Child Health and Human Development



The Endocrinology and Reproduction Research Branch, Intramural

Research program, National Institute of Child Health and Human

Development, is seeking organizations with the capability to provide

freshly prepared and purified rat Leydig cells from rat testis.  This

program also requires the provision of short-term Leydig cell

cultures from adult rats, long-term cultures from rat fetal testes,

Nb2 lymphoma cells, and of established mouse tumor Leydig cell lines.

In addition, it requires the preparation of cell fractions and of

well-characterized gonadotropin and prolactin receptor preparations

from luteinized rat ovaries, testes and SF9 cells overexpressing the

LH receptor.  The successful contractor must have the capability of

delivering the viable and hormone-responsive Leydig cell preparations

before 8:00 a.m. within one hour of completion of the preparation

procedure three times a week to the National Institutes of Health,

Bethesda, Maryland.  This announcement is a new solicitation, but not

a new project.  The issuance of this Request for Proposals (RFP) will

be on or about April 28, 1995, and proposals are due by 4:00 p.m.

(local time), June 30, 1995.



INQUIRIES



All requests must cite RFP number NICHD-IRP-95-15.  All sources who

consider themselves qualified are encouraged to submit a proposal.

This advertisement does not commit the Government to make an award.

Those organizations interested in responding must request the full-

text version of the RFP, in writing, from:



Mrs. Lynn Salo

Contracts Management Branch

National Institute of Child Health and Human Development

Executive Building, Room 7A07

6100 Executive Boulevard MSC 7510

Bethesda, MD  20892-7510

Telephone:  (301) 496-4611

FAX:  (301) 402-3676



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