PERFORMANCE OF RADIOIMMUNOASSAYS AND RADIOIODINATIONS NIH GUIDE, Volume 24, Number 1, January 13, 1995 RFP AVAILABLE: NICHD-IRP-95-12 P.T. 34 Keywords: METHODOLOGIES/PROCEDURES Bioassay RESEARCH RESOURCES National Institute of Child Health and Human Development The National Institute of Child Health and Human Development (NICHD) has a requirement for immunoassay, bioimmunoassay, and radioiodination services. These services will support ongoing research conducted by NICHD's Intramural Research Program and other Intramural Investigators of NIH. The tests will be quantitated utilizing radioisotopic and fluorescent markers. Immunoassays will routinely be developed that are capable of detecting picomole or femtomole concentrations of antigen. Custom iodinations will be performed in support of such assays with 125I using standard chloramine-T, lactoperoxidase, and Bolton-Hunter reagents, and electrolytic and iodogen methodologies. Among the purification procedures employed will be Sephadex gel, ion exchange, concanavalin- A, electrophoresis, and high pressure liquid chromatography. Specific activity, binding efficiency with excess antibody trichloroacetic acid precipitation, and chromatographic elution patterns will be included with each preparation. The performance of immuno-and in vitro bioimmunoassays for hormones and certain drugs in human, monkey, rat, and mouse shall be required. A minimum of 50,000 assays shall be performed for each year of the contract(s). This number may be adjusted depending on the number of actual awards. The laboratory of the successful Offeror(s) must be certified by the Nuclear Regulatory Commission. In addition, the Contractor must be available for frequent discussions and monthly meetings with the Project Officer and the Intramural staff. The RFP will be available on or about January 20, 1995. INQUIRIES All responsible sources are encouraged to submit a proposal. Several awards will be considered. Organizations desiring a copy of the RFP should send a self-addressed mailing label along with their written requests to: Mrs. Dorothy McKelvin Office of Grants and Contracts National Institute of Child Health and Human Development 6100 Executive Boulevard, Room 7A07 MSC 7510 Bethesda, MD 20892-7510 .
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