NICHD TRANSGENIC MOUSE DEVELOPMENT FACILITY NIH GUIDE, Volume 23, Number 14, April 8, 1994 P.T. Keywords: National Institute of Child Health and Human Development The National Institute of Child Health and Human Development (NICHD) Transgenic Mouse Development Facility (NTDF) is a research resource that develops transgenic mice for investigators requiring these services. It was established to provide reliable and affordable transgenic mice primarily to biomedical research grantees. Specifically, the NTDF supports basic biomedical research by providing investigators with the following customized services: o Analyzing constructs for microinjection. o Microinjecting constructs into fertilized one-cell mouse eggs and reimplanting into pseudopregnant recipient females. o Testing potential founders for DNA integration. o Producing at least two integration-positive transgenic mice. o Fast turnaround time (12-14 weeks) Applications for services should be requested from the Director, NTDF. Completed applications may be submitted anytime to the NICHD Project Officer for review. Review is completed within two weeks of receipt. All applications are handled confidentially and those constructs approved are microinjected at the NTDF. The Investigator is charged a non-refundable fee of $500 per construct for initial analysis. An additional fee of $1500 per construct is assessed if two or more integration-positive transgenic mice are produced. The NTDF is supported by a contract award from the National Institute of Child Health and Human Development, NIH. INQUIRIES Direct programmatic inquiries regarding this research resource to: Allan Lock, D.V.M. NTDF Project Officer National Institute of Child Health and Human Development Building 6100, Room 4B01 Bethesda, MD 20892 Telephone: (301) 496-5541 Direct requests for applications and resource inquiries to: Mark Swanson, Ph.D. or Mr. Frederick Huntress NICHD Transgenic Mouse Development Facility 303B College Road East Princeton, NJ 08540 Telephone: (609) 520-0300, Ext. 183 FAX: (609) 520-9864 .
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